The Test match in the multiformat 2025 Women’s Ashes series in Australia will be a day-night game at the 100,000-capacity Melbourne Cricket Ground.
The Test returns to a four-day affair after it was extended to five days during the 2023 series in England.
The pink-ball game, which starts on January 30, will also end the multi-format series for the first time since it was introduced in 2015.
Australia retained the Ashes with an 8-8 draw last year in England.
The series will also consist of three one-day internationals and three T20s – each worth two points.
The Test is worth four points.
The ODIs will start the series from January 12, before the T20s begin on January 20.
Australia won the Test in 2023, but England claimed both white-ball series 2-1.
England last won the Women’s Ashes in 2014, with a 10-8 victory down under.